Background: Injuries involving skeletal muscle disturb millions across the globe. Platelet-rich plasma (PRP) is encouraging strategy for improving tissue healing. Nigella sativa (NS) have broad-spectrum therapeutic properties. Aim: The research assesses the potential beneficial impact of PRP and NS on muscle injury. Materials and Methods: 112 animals were allocated into five experimental groups: Group I, The control group. Group II: administered only glycerol. Group III: administered glycerol then PRP intramuscularly. Group IV: administered NS orally for 21days then glycerol. Group V: given NS orally for 21days then glycerol then PRP. Half of the rats were euthanized 7 days after glycerol injection (subgroups a) and the other half 14 days after injection (subgroups b). H&E, Masson’s trichrome staining, myogenin immunostaining, and ultrastructural analysis were performed on muscle sections. Results: Group II exhibited destruction of myofibers and significant (P