It has been the first trail to do intra-articular injection in cattle and buffalo as far as we know so, the purpose of the present study was to state the site of intra-articular injection of fetlock, pastern and coffin joints in these animals. Also to provide a detailed computed tomography (CT) and cross sectional anatomic reference of the normal metacarpus and digits for the cattle and buffalo, as well as to compare between metacarpus and digits in these animals to outstand a basis for diagnosis of their affections. Advantages, including depiction of detailed cross-sectional anatomy, improved contrast resolution and computer reformatting, make it a potentially valuable diagnostic technique. The fore limbs of ten healthy adult cattle and buffalo were used. Clinically relevant anatomic structures were identified and labeled at each level in the corresponding images (CT and anatomic slices). CT images were used to identify the bony and soft tissue structures of the metacarpus and digits. The knowledge of normal anatomy of the cattle and buffalo metacarpus and digits would serve as initial reference to the evaluation of CT images in these species. Intra-articular injection was done to clarify the joint of the digits.