This study investigated the effects of dietary antimicrobial peptides (AMPs) on production performance, egg quality, immune function, antioxidant capacity, and intestinal microbiota in late-laying hens. A total of 576 hens (55 weeks old) were randomly assigned to three dietary treatments with eight replicates per treatment and 24 hens per replicate: CON (basal diet), AMP100 (basal diet + 100 mg/kg AMPs), and AMP200 (basal diet + 200 mg/kg AMPs). Dietary AMPs supplementation significantly increased the egg-laying rate (p