Background: Ophthalmological trauma is a critical public health issue that may cause
vision loss, mono-ocular visual disability and non-congenital mono-ocular blindness in children
because of their immature motor skills and natural curiosity. Aim of study: Was to evaluate the
effect of designed guidelines on knowledge and practice of mothers in caring for their children with
ophthalmological trauma. Design: A quasi- experimental design was utilized. Setting: The study
was accomplished at inpatient department at Specialized Ophthalmology Center of Benha
University Hospital. Sample: A purposive sample of 80 mothers who had children having
ophthalmological trauma and attended the inpatient department of Specialized Ophthalmology
Center. Tools of data collection: Three tools have been utilized to conduct the study. I: Knowledge
of mothers structured questionnaire. II: Medical and social characteristics of children and III: Care
of ophthalmological trauma observational checklists which include eye care, application of hot and
cold compresses, eye ointment application, eye drops instillations, and eye irrigation. Results: At
pre/ post-guidelines, a strong positive statistical correlation was noticed between mothers' total
knowledge and their total practice scores. Conclusion: The research hypothesis was accepted,
where most of the studied mothers had adequate knowledge and satisfactory practice following
implementation of designed guidelines relative to pre designed guidelines with strongly significant
statistical improvement. Recommendations: The nursing roles should focus on educating mothers
on how to provide proper care for children with ophthalmological trauma.