Background
Ultrasound imaging has become an invaluable tool in veterinary medicine, particularly in guiding injections and visualizing soft tissue structures. Recently, ultrasonography has been used in camel practice to detect cosmetic fillers, particularly in aesthetic evaluations. However, previous applications lacked a controlled experimental background. This study is the first to experimentally assess the effectiveness of ultrasound in detecting and localizing botulinum toxin (BOTOX) injections in camels, using two different doses, from the time of injection until the toxin’s effect becomes undetectable.

Objective
The objective of this study was to assess the efficacy of ultrasound in detecting and localizing botulinum toxin injection sites in camels’ lips.

Methods
This study involved 18 adult Arabian dromedary camels (9 Magateer and 9 Majaheem breeds) from the Camel Research Center, King Faisal University. The camels, aged 4.3 ± 1.3 years and weighing 405.5 ± 20.6 kg, were randomly assigned to three treatment groups (6 camels each). Treatments included 100 IU and 200 IU of botulinum toxin type A, and a control of 5 ml sterile saline, administered to the upper and lower lips. Morphological changes, inflammation, and lip thickness were assessed weekly for two months. Ultrasound examinations and hematological and biochemical analyses were conducted at specified intervals. Data were analyzed using two-way repeated-measures ANOVA and Tukey’s test, with significance set at p