مها ممدوح عبد الجواد يوسف خونده
أستاذ مساعد
Exploring genetic polymorphisms and transcript levels of antioxidant and metabolic markers for prediction and monitoring diarrhea in Holstein dairy calves
ACTAActa Agriculturae Scandinavica, Section A — Animal Science • 2023
معلومات البحث
المؤلفون
Ahmed Ateya, Mona Al-Sharif, Salah H. Faraj, Mohamed Abdo, Liana
Fericean, Ioan Banatean-Dunea, Salam J. Hammad, Maha Mamdouh, Hayat
Fayed, Marawan A. Marawan & Walaa S. Raslan
الكلمات المفتاحية
Dairy calves; genetic
polymorphisms; transcript
level; diarrhea
المجلة العلمية
ACTAActa Agriculturae Scandinavica, Section A — Animal Science
الناشر
taylor
المجلد
53
العدد
1904
الصفحات
14-19
publication.type
International
رابط البحث
Open Link
المواد المرفقة
Maha Mamdouh Abdel_Gawad Youssef_Exploring genetic polymorphisms and transcript levels of antioxidant and metabolic markers for prediction and monitoring diarrhea in Holstein dairy ca-1.pdf
الملخص
The objective of this study was to investigate the genetic polymorphisms and transcript levels of
antioxidant and metabolic markers for diarrhea monitoring in Holstein dairy calves. For DNA and
RNA extraction, blood samples were taken from 100 female calves (50 with diarrhea and 50
appearing healthy). DNA sequencing and real time PCR for and metabolic genes were
performed to identify genetic variants linked to diarrhea susceptibility. Results revealed that
there were nucleotide sequence variations in the investigated genes between healthy and
diarrheal calves. Keap1, HMOX1, CMPK2, ASPG, FPGT, TNNI3 K, and LPCAT1 were considerably
more highly expressed in diarrheic calves than resistant ones. The Nrf2, PRDX2, and PRDX6
genes, however, produced a different pattern. This study provides a practical control strategy to
lessen newborn diarrhea in Holstein dairy calves and emphasizes the significance of nucleotide
variations along with antioxidant and metabolic gene expression patterns as proxy markers of
diarrhea susceptibility/resistance.
antioxidant and metabolic markers for diarrhea monitoring in Holstein dairy calves. For DNA and
RNA extraction, blood samples were taken from 100 female calves (50 with diarrhea and 50
appearing healthy). DNA sequencing and real time PCR for and metabolic genes were
performed to identify genetic variants linked to diarrhea susceptibility. Results revealed that
there were nucleotide sequence variations in the investigated genes between healthy and
diarrheal calves. Keap1, HMOX1, CMPK2, ASPG, FPGT, TNNI3 K, and LPCAT1 were considerably
more highly expressed in diarrheic calves than resistant ones. The Nrf2, PRDX2, and PRDX6
genes, however, produced a different pattern. This study provides a practical control strategy to
lessen newborn diarrhea in Holstein dairy calves and emphasizes the significance of nucleotide
variations along with antioxidant and metabolic gene expression patterns as proxy markers of
diarrhea susceptibility/resistance.