Four wheat cultivars, Gemmeiza10, Misr1, Sakha93, and Giza168 were used in this study as well as the hybrids
resulted between all the studied four cultivars. Misr1 Sakha93 resulted hybrids were identified using salinity
primers Na+/H+ antiporter gene amplification followed by sequencing while Giza168 Gemmeiza10 resulted
hybrids were identified by drought primers DREB2 gene amplification followed by sequencing. The resulted
sequences were compared with those available on NCBI website database through the BLAST bioinformatics
tool. The obtained data showed that, the base size of Na+/H+ antiporter gene was 400 bp while the base size of
DREB2 gene was 200 bp. the phylogenetic analysis showed that Na+/H+ antiporter gene obtained sequence was
related to four accession numbers from gene bank. In addition, phylogenetic analysis exhibited an amount of
genetic change on the root of clades 0.0020, where the Na+/H+ antiporter gene -based phylogeny tree included
four clades. The clades grouping had low support (bootstrap value between 6-8%). Also, the obtained DREB2
gene sequences were aligned with six accession numbers in the NCBI website database as shown from the
phylogeny tree. The phylogenetic tree deducted from the sequence comparison of DREB2 gene region showed
that the length of branch that represents an amount of genetic change of 0.0020, where it’s based phylogeny tree
included four clades. The clades grouping had low support (bootstrap value between 8-12%)