Background
Prolyl-4-hydroxylase subunit beta (P4HB) and special AT-rich region-binding
protein-1 (SATB1) have been implicated in tumorigenesis and progression in
many cancers, but their significance in bladder urothelial carcinoma remains to
be elucidated. This study aimed to investigate the correlation and prognostic value
of P4HB and SATB1 expression along with clinicopathological features in bladder
transitional-cell carcinoma.
Patients and methods
This is a retrospective, selected, controlled study carried on 50 cases of
bladder urothelial carcinoma to detect the expression of P4HB and SATB1
immunohistochemistry and statistical correlation with various clinicopathological
parameters, including molecular subtypes.
Results
Prolyl-4-hydroxylase subunit beta (P4HP) is highly expressed in 48% of the study
cases. P4HP expression was significantly associated with size of the tumor
(P=0.002), muscle invasion (P=0.000), the grade of tumor (P=0.000), and the
depth of invasion of the primary tumor (T) (P=0.000). High SATB1 expression
was detected in 46% of the study cases. A significant association was detected
between SATB1 expression and molecular subtypes (P=0.001), size of the
tumor (P=0.004), histopathological type (P=0.024), muscle invasion (P=0.000),
the grade of tumor (P=0.000), and the depth of invasion of the primary tumor
(T) (P=0.000). Receiver operating characteristic curve was carried on for P4HP
and SATB1 in relation to molecular classification and showed that SATB-1 has
the highest sensitivity (75%) and specificity (70%) in discrimination between
luminal versus nonluminal subtypes with significant relation (P=0.01). There was
significant association between P4HP and SATB1 expression in bladder urothelial
transitional-cell carcinoma (P=0.000).