Objective: study the effect of Zinc oxide nanoparticles on shelf life extension of chicken meat fillets and
elimination of Campylobacter jejuni.
Design: Experimental study.
Samples: Nine kg of fresh chilled chicken fillets divided into 45 chicken fillets (each sample weight 100-
120 g) were collected from various poultry shops in Benha City.
Procedures: Campylobacter jejuni was intentionally inoculated into broiler breast fillets, followed by the
application of zinc oxide nanoparticles (ZnO NPs) suspension with varying concentrations (5 mM and 10
mM) with 3 different sizes (25 nm, 50 nm, and 75 nm) for each concentration and storage at 4 °C.
Results: The results showed that ZnO suspensions had a strong inhibitory effect on Campylobacter jejuni
growth after 8 days in the refrigerator at 4 °C. Among the concentrations used of 5 and 10 mM ZnO NPs,
the higher antibacterial activity with a subsequent marked reduction in Campylobacter jejuni was
concentration-dependent. Additionally, ZnO NPs had a significant impact on the virulence genes of
Campylobacter jejuni (cdtA, cdtB, and cdtC), especially at diameters of 25 and 50 nm on one side and 10
mM on the other side. ZnO NPs have the strongest impact due to their tiny size.
Conclusion and clinical relevance: ZnO NPs can eliminate Campylobacter jejuni from chicken meat and
extend the shelf life of chicken fillets without affecting its sensory characteristics.