Clostridium perfringens is gram positive bacterium, wide spread in environment causing various diseases in ani
mals and human. The current study was conducted to indentify the genetic identity of C. perfringens isolates from
lambs from Egypt. Using specific primers amplifying genes associated to the toxins produced by C. perfringens,
multiplex PCR was used to confirm C. perfringens in 87 out of 140 samples were collected from diseased and
suspected lambs. The isolates were classified as type A in 49.4%, type B in 31.1% and type D in 19.5% of isolates.
The phylogenetic analysis for the partial sequences of C. perfringens strains based on plc gene, cpb gene and etx
gene obtained in the present study showed high degree of similarity with other sequences of C. perfringens strains
in GenBank, isolating from sheep from Egypt and other countries. According to the findings, lambs with
enterotoxaemia more frequently have C. perfringens type A and an efficient hygienic control program is necessary to reduce the infection spreading among susceptible animals.