PASTEURELLA multocida infection has serious complications for both human and animal
health. In bovines, it is the predominant cause of fatal pneumonia, with a mortality rate
up to 100%, especially in severe undiagnosed cases. Therefore, this study was conducted for
molecular identification and characterization of P. multocida in nasal swabs and pneumonic lung
tissue in cattle calves less than one year suffered from pneumonia and respiratory disorders.
The detection of P. multocida was higher in pneumonic lung tissue (40%) than in nasal swabs.
Only 37 out of 109 collected samples were positive for P. multocida using bacteriological culture
and those samples were confirmed by PCR targeting Kmt1 gene. The phylogenetic analysis of
local P. multocida isolate revealed close relation with other P. multocida strains from bovine
from Egypt, China, USA and Canada. Consequently, establishing a potent epidemiological
surveillance program is necessary to decrease the spreading of disease and the economic losses among fattening calves.