Intraventricular hemorrhage (IVH) is a major neurological complication responsible for preterm neonates' morbidity and mortality. In the first 72 hours of life, nurses have acritical role in maintaining bundled practices for intraventricular hemorrhage prevention. The study aimed to assess preventive care bundle guidelines for nurses on the incidence of intraventricular hemorrhage among preterm neonates who were mechanically ventilated. This study was carried out at Neonatal Intensive Care Unit of Specialized Pediatric Hospital at Benha city. Sample of the study was convenient sample for nurses and purposive sample for preterm neonates who consisted of 60 preterm neonates on mechanical ventilation and 60 nurses who cared for them at previous setting. Tools of data collection included four tools, (1) A structured Interviewing Questionnaire Sheet, (2) Medical record of preterm neonates, (3) Intraventricular hemorrhage grading scale, (4) preventive care bundle checklist. The main results of this study revealed high statistical significant improvement in the studied nursesʹ total level of knowledge and total practice post- preventive care bundle guidelines implementation. Additionally, there was a negative correlation between total nurses' knowledge, their practice and IVH incidence pre and post-neonatal care bundle guidelines implementation