Background and rational: Interleukin-10 (IL-10) is a major
anti-inflammatory and immunosuppressive cytokine that
plays an important role in regulation of immune system. Its
activity may be important for clinical outcome of diabetic
kidney disease (DKD). This study aimed to evaluate the
association between the genotypic and allelic frequencies of
the IL-10 (-1082G/A) polymorphism and the risk of
developing DKD in a group of Egyptian patients.
Patients and Methods: The IL-10 (1082) G/A gene
polymorphism was detected using PCR-RFLP in 50 subjects,
40 type 2 diabetic patients with diabetic kidney disease
(DKD) and 10 diabetics without DKD as a control subjects.
The subjects were divided in to 3 groups; (Group I), included
20 albuminuric patients, with A/C ratio > 30 mg/g, (Group
II), included 20 non albuminuric patients, with A/C ratio <
30 mg/g, and (Group III), 10 type 2 diabetic patients as
controls without DKD.
Results: IL-10 gene variants showed statisticaly significant
association in the different studied groups and the most
frequent genotype in patients with DKD was GG genotype.
Logistic regression analysis revealed that both urine albumin
creatinine ratio (UACR) and e GFR were significant
predictors of GG genotype, but UACR was much stronger
predictor. (p=0.005 and 0.019) respectively.
Conclusion: The IL-10 (1082) G/A gene polymorphism
might be associated with the risk of development of DKD in
the studied patients. Moreover, the mutant G allele of the
polymorphism was associated with an increased risk of DKD
compared to the wild A allele.