Leaders with high emotional intelligence play a key role in leadership Practice and lead to effective organizational climate. The study aimed to assess the effect of emotional intelligence educational program on the leadership practice of head nurses. Research design: A quasi-experimental pre and post-test and follow-up (after 3 months) design was utilized to conduct this study. Setting: Benha University Hospital in inpatient units. Subject: A convenience sample (57) of head nurses and a random sample (150) of staff nurses available during the time of the study. Tools of data collection: Three tools were used in the present study for data collection: An emotional Intelligence Knowledge questionnaire, an Emotional Intelligence questionnaire level, and a Leadership Practice Inventory. Results: There was a highly significant improvement in head nurses’ total emotional intelligence knowledge among head nurses, head nurses’ total emotional intelligence level, and total leadership practice as reported by head nurses and staff nurses throughout the program phases. Moreover, there was a high statistically significant correlation between head nurses’ total emotional intelligence knowledge and levels and total leadership practice as reported by head nurses and staff nurses throughout the program phases.