The objectives of this study were, firstly, to conduct genetic characterization of Egyptian Arabian horses based on
14 TKY microsatellite markers, secondly, to investigate the powerfulness of these 14 TKY markers for parentage assignment of
Arabian horses. A total of 101 horse samples including (Arabian = 71, Thoroughbred = 19 and Nooitgedacht = 11) were analysed
by 14 TKY microsatellite markers. The PCR products were electrophoresed on Genetic analyzer 3500 with the aid of Liz standard.
The basic measures of the allele’s size and genetic diversity were computed using bioinformatics software. The polymorphism
of the TKY markers across the Arabian population showed moderate values for genetic diversity parameters; number of allele
(NA) =8.143, effective number of allele (Ne) = 3.694, observed heterozygosity (HO) = 0.599, expected heterozygosity (HE) = 0.691,
polymorphic Information Content (PIC) = 0.636 and Inbreeding coefficient (FIS) = 0.128. The combined probability of exclusion
(CPE) value of the 14 TKY microsatellite loci of our Arabian horses was 0.9999. The results from current study confirm the applicability and efficiency of TKY microsatellite panel for evaluating the genetic diversity and parentage assignment of Egyptian Arabian
horses