Postpartum deep venous thrombosis is a leading cause of maternal mortality and can carry significant long-term morbidity. Aim: was to investigate effect of implementing evidence-based nursing practices guidelines on prevention of deep venous thrombosis among postpartum women. Design: A quasi-experimental research design. Sample: A purposive sample of two groups (50 women in study group and 50 women in control group). Setting: This study was conducted at antenatal clinic and postpartum unit of Obstetrics and Gynecological Department in Benha University Hospital, Qaluobia, Egypt. Tools: Four main tools were utilized. Tool (I): A structured interviewing questionnaire. Tool (II): Deep vein thrombosis risk factors assessment sheet. Tool (III): Women’s compliance with the DVT preventive measures. Tool (IV): Deep vein thrombosis assessment sheet. Results: There was highly statistically significant difference between study and control groups regarding their knowledge about DVT at post-intervention compared to pre- intervention. Additionally, there was a highly statistical significance difference among both groups post-intervention regarding to their compliance with DVT preventive measures. Moreover, a significant reduction in the occurrence and manifestations of DVT in the study groups compared to control groups with statistically significant difference post-intervention