One of the top five leading causes of women deaths worldwide is puerperal sepsis., preventive care bundle are the best nursing interventions based on evidence applied in clinical practice for prevention puerperal sepsis. Aim: The present research aimed to evaluate the effect of utilizing care bundle on prevention of puerperal sepsis among post-natal women. Design: A quasi-experimental research design (study/control group, pre/post-test) was utilized. Sample: A purposive sample of 100 women (pregnant women at 36 gestational weeks and followed immediately after delivery). Setting: The present research conducted at the out- patient clinic of obstetrics & gynecology department and postnatal room (recovery room) at Benha University Hospital. Tools: three tools utilized included: I- A structured interview questionnaire, II-part one -knowledge assessment questionnaire, part two-care bundle practices scale, III-the sepsis in obstetric score sheet.