Bovine viral diarrhea virus (BVDV) infections continue to cause significant losses in cattle population. Better isolation of BVDV may influence in significant prevention and control of BVDV. This study was designed to isolate and identify the circulating BVDV in Egyptian cows and heifers during 2019. The isolated virus was identified and detected by immune florescent assay and immune peroxidase technique followed by RT- PCR that confirmed circulation of non cytopathic BVDV-1 in Egyptian cows and heifers. Further molecular analysis is recommended to determine the molecular epidemiology of BVD isolate for updating vaccine strain as a successful preventive strategy. It is obvious from the obtained result that immune peroxidase technique and immune florescent assay is highly sensitive and specific tool for detection of cytopathic and non cytopathic BVDV; More over RT-PCR is a highly sensitive tool for genotyping of BVDV. Further molecular analysis is recommended to confirm these finding and determine the molecular epidemiology of BVD-1 isolate and update the nature of the future vaccine for successful preventive strategies