The purpose of this study was to investigate the effect of fatigue on balance and chest expansion in normal adults. Materials and methods: thirty eight normal subjects from both genders were participated in this study. They were recruited from faculty of physical therapy students and workers. Their ages were ranged between 18-25 years. They were grouped into one group. Biodex lab was used to measure balance pre-post fatigue. Maximum voluntary ventilation was measured by spirometer. Chest expansion was measured by tape measurement. Results: A statistical significant decrease was found in balance, maximum voluntary ventilation and upper chest expansion. Conclusion: Fatigue affected on balance and chest expansion.