Salwa safwat abd elati shalan
Asst. Lecturer
Effect of Pomegranate Peel and Grape Seed Extract on Carcass Traits, Biochemical Parameters, and Immune-Related Gene Expression in Rabbits
• 2026
Publication Information
Authors
Salwa S. Shalan1
, Sherif I. Ramadan1
, Hadeel S. El-Qaliouby1
, Basant Mohamed Shafik1
, Eman Ahmad Sallam1
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publication.type
Local
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Abstract
This study assessed the effects of dietary supplementation with pomegranate peel extract
(PPE) and grape seed extract (GSE) on carcass traits, biochemical parameters, hematological
indices, and immune gene expression in Alexandria and V-line rabbits. A total of 108 weaned
male rabbits (54 per breed) were randomly allocated to three dietary treatments: control, PPE
(250 mg/kg), and GSE (250 mg/kg) for 56 days. GSE supplementation significantly increased
live body weight in V-line rabbits (2030 g) compared with the control (1525 g), while PPE
showed moderate improvements (1772 g). organ weights remained unaffected, with no
adverse effects observed on blood biochemical parameters or hematological indices, further
confirming the safety of both extracts. PPE improved lipid profiles in V-line rabbits by
increasing HDL (26.60 mg/dL) and reducing LDL cholesterol, though triglycerides were
elevated (P = 0.00) in Alexandria rabbits. Hematological analysis showed reduced (P = 0.02)
white blood cell counts in supplemented groups, indicating decreased systemic inflammation.
Gene expression analysis revealed that both extracts significantly upregulated antiinflammatory IL-10 (4.170 in V-line GSE) and pro-inflammatory TNF-α (3.048 in V-line
GSE) compared to control (≈1.000), suggesting balanced immunomodulatory effects. In
conclusion, dietary supplementation with PPE and GSE at 250 mg/kg enhanced growth
performance and modulated immune responses in rabbits with breed-specific variations,
supporting their potential as functional feed additives in sustainable rabbit production systems.
(PPE) and grape seed extract (GSE) on carcass traits, biochemical parameters, hematological
indices, and immune gene expression in Alexandria and V-line rabbits. A total of 108 weaned
male rabbits (54 per breed) were randomly allocated to three dietary treatments: control, PPE
(250 mg/kg), and GSE (250 mg/kg) for 56 days. GSE supplementation significantly increased
live body weight in V-line rabbits (2030 g) compared with the control (1525 g), while PPE
showed moderate improvements (1772 g). organ weights remained unaffected, with no
adverse effects observed on blood biochemical parameters or hematological indices, further
confirming the safety of both extracts. PPE improved lipid profiles in V-line rabbits by
increasing HDL (26.60 mg/dL) and reducing LDL cholesterol, though triglycerides were
elevated (P = 0.00) in Alexandria rabbits. Hematological analysis showed reduced (P = 0.02)
white blood cell counts in supplemented groups, indicating decreased systemic inflammation.
Gene expression analysis revealed that both extracts significantly upregulated antiinflammatory IL-10 (4.170 in V-line GSE) and pro-inflammatory TNF-α (3.048 in V-line
GSE) compared to control (≈1.000), suggesting balanced immunomodulatory effects. In
conclusion, dietary supplementation with PPE and GSE at 250 mg/kg enhanced growth
performance and modulated immune responses in rabbits with breed-specific variations,
supporting their potential as functional feed additives in sustainable rabbit production systems.