Benha University

Ahmed Abdel-Khalek Salem Salem Nayl

Professor

Faculty of Agriculture MICROBIOLOGY

Wild Strawberry-like Flavor Produced by the Fungus Wolfiporia cocos, Identification of Character Impact Compounds by Aroma Dilution Analysis after Dynamic Headspace Extraction.

J. Agric. Food Chem. 2021, 69, 47, 14222–14230 • 2021
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Publication Information
Authors 23. Svenja Sommer, Marco A. Fraatz, Julia Büttner, Ahmed A. Salem, Martin Rühl and Holger Zorn
Keywords aroma dilution analysis, dynamic headspace, side stream, black currant pomace
Journal J. Agric. Food Chem. 2021, 69, 47, 14222–14230
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publication.type International
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Abstract
Brown-rot fungi are particularly suitable for the sustainable and cost-efficient biotechnological production of natural flavors. In this study, Wolfiporia cocos was employed for the fermentation of European black currant pomace supplemented with aspartate in surface culture to produce a flavor reminiscent of wild strawberries. Aroma dilution analysis (ADA) by means of dynamic headspace extraction (DHS) was employed as a suitable technique for solid samples. The character impact compounds were quantified by stable isotope dilution analysis (SIDA) and standard addition and validated by recombination experiments. (R)-linalool (1,879 µg kg-1, ADA 211), methyl anthranilate (2,206 µg kg-1, 210), 2 aminobenzaldehyde (771 µg kg-1, 25), and geraniol (138 µg kg-1, 25) were identified as key aroma compounds. Recombination experiments demonstrated that the combination of the four analyzed compounds was responsible for the odor impression reminiscent of wild strawberries.
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