Benha University

Alaa El-Sayed Ahmed Ahmed Amin

Prof. Emeritus

Faculty of Science Chemistry

New colorimetric methods for the determination of indapamide and its formulations.

• 2001
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Publication Information
Authors H.M. Saleh, A.S. Amin and M. El-Mammli
Keywords Not Available
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publication.type International
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Abstract
Two simple and sensitive methods for the determination of indapamide in pure and in dosage forms are developed. These methods are based on the oxidation of indapamide with iron(III) in acidic medium. The liberated iron(II) reacts with 1,10-phenanthroline (Method A) and the ferroin complex is colorimetrically measured at λmax 509 nm against reagent blank. Method B is based on the reduction of Fe(III) by the drug. Iron(II) forms a colored complex (λmax 522 nm) with 2,2′-bipyridyl. Optimization of the experimental conditions is described. Beer’s law is obeyed in the concentration range 1.0–12 µg ml−1 and 4.0–18 µ g ml−1 for A and B, respectively. The apparent molar absorptivity and Sandell sensitivity for method A is 3 × 104 L mol−1 cm−1 and 0.0188 µ g cm−2, while for method B is 2.3 × 104 L mol−1 cm−1 and 0.0159 µg cm−2. The detection and quantification limits are calculated. The developed methods are applied successfully for the determination of indapamide in pure and in tablet form without interference from common excepients. 

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