Great effort s have been est ablished to promote healthy aging and prevent illnesses connected to aging. The prevalence of elderly population increases and this will be associated with an increase in disability and illness. This study analyzed renal aging process following administration of D-galactose (120 mg/kg/d ip for 8 weeks), and the potential protective effects of vitamin D administration (1,0 0 0 and 10,0 0 0 IU/kg/d by oral gavage for 8 weeks) in animals with D-galactose induced aging. The renal function tests (serum urea and creatinine, creatinine clearance, and 24 hours urinary albumin excretion levels), oxidative stress (MDA, TCA), renal tissue expression of TNF α, HIF α, Adropin, MAPK, eNOS, and VEGF as well as histopathological and EM alterations were assessed. D-galactose administration resulted in noticeable changes in renal histopathology, deteriorated renal function tests, elevated oxidative stress and inflammation, and significantly diminished Adropin, MAPK, eNOS, HIF α and VEGF expression. Vitamin D effectively reversed these alterations and enhanced histopathological and EM ultrastructure changes triggered by D-galactose administration.