Background: Hospital-acquired infections are the major concern of the health care system and
agencies particularly in the isolation department. Nurses have the key and most important role in
applying infection control measures in isolated departments. Aim of study: This study aimed to
evaluate the effect of educational program on nurses' performance and compliance regarding infection
control measures in isolated departments. Design: Quasi-experimental research design was utilized to
conduct the aim of this study. Setting: The study was conducted in the isolation departments at Benha
University Hospital. Sample: A convenient sample of all available nurses (60) from both sexes who
are working at the previous mentioned setting. Tools: Three tools are used Tool I: A Self –
administered questionnaire Tool II: Observational checklist Tool III: ـــــــ The Isolation Precautions
Compliance Scale. Results: 18 % of the studied nurses had satisfactory level of the total knowledge
at preprogram implementation which improved to 85% immediately post and slightly decreased to
78.3% after 3 months post program implementation. It showed statistically highly significant
improvement between total practice and compliance immediately post and after 3 months of program
implementation as compared with pre program p (0.001**). Conclusion: Educational program
effectively improved the studied nurses’ knowledge and practice regarding infection control measures
with a highly statistical significance correlation between nurses performance and their compliance in
the isolation department. Recommendation: Providing continuous educational or training program
for nurses regarding occupational hazards and especially on protective measures.