Hypercholesterolemia can be defined as the presence of high plasma cholesterol levels, with normal plasma triglycerides, as a consequence of the rise of cholesterol and apolipoprotein B (apoB)-rich lipoproteins, called low-density lipoprotein (LDL)and characterized by very high levels on the blood cholesterol which is a risk factor of cardiovascular health. Medicines which formed from chemical compounds cause serious side effects that cause an imbalance in the body’s functions. Therefore, using of natural substances may be an innovative method for the treatment of diseases, as they do not have any side effects on human health. Bee venom can be used as a drug due to its beneficial effects on many disorders, such as neurological, cardiovascular, hematological, musculoskeletal, and dermatological disorders. In the present work, we found that treatment of hypercholesterolemic rats with bee venom (BV) revealed significant increases in Hb, Hct, and RBCs count and showed a significant increase in PaO2, % O2 saturation, O2 carrying capacity. These results suggest that BV can enhance respiratory functions of blood and can be used in respiratory diseases to enhance the respiration and hematological parameters. Also, BV could be used as a prophylactic agent (food protector) to protect us from the harmful effects of cholesterol