Background: A tracheostomy is one of the most common procedures performed in pediatric intensive care unit for critically ill children requiring prolonged mechanical ventilation for acute respiratory failure and for airway issues. Aim of study: Was to evaluate effect of educational program on nurses' performance regarding care of children with tracheostomy. Design:A quasiexperimental design was used in the current study. Settings: The present study was conducted at Pediatric Intensive Care Unit in Benha University Hospital and Pediatric Intensive Care Unit & Neonatal Surgical Care Units in Benha Specialized Pediatric Hospital. Subjects: A convenient sample of (70) nurses who working at the previously mentioned settings were included in the study and a purposive sample of (48) child who had tracheostomy. Tools of data collection: Two tools were used. Tool (1): A structured interviewing questionnaire sheet to gather data in relation to characteristics of the studied subjects and nurses' knowledge regarding care of children with tracheostomy. Tool (II): Observational checklists to assess nurses' practices in care of children with tracheostomy. Results: Nearly three quarters of the studied nurses had good knowledge level in post program implementation. Also, the majority of the studied nurses had competent practice in post program implementation regarding care of children with tracheostomy. Conclusion: Educational program was effective in improving nurses' performance regarding care of children with tracheostomy. There was a positive correlation between nurses’ total level of knowledge and practices pre and post program implementation. Recommendation: Conducting regular educational program and workshops for nurses regarding care of children with tracheostomy.