Background: Puerperal sepsis is one of the major causes of preventable maternal morbidity and mortality, a common pregnancy-related condition that could eventually lead to obstetric shock or even death in some cases. Aim of the study: The study aimed to evaluate the effect of an educational program on knowledge and self-care practices of pregnant women regarding prevention of puerperal sepsis. Design: A quasi-experimental study design (one group: time series quasi-experimental design) was used to fulfill the aim of study. Setting: The study was conducted at Obstetrics and Gynecological outpatient clinic affiliated to the Benha University Hospital. Sample: A purposive sample included (120) pregnant women. Tools of data collection: Three tools were used, tool I: A structured interviewing questionnaire which included two parts: General characteristics of pregnant women and obstetrical history, tool II: Pregnant women’s knowledge assessment sheet and tool III: Pregnant women’s self-care practices assessment sheet Results: There was a highly statistical significant improvement among pregnant women level of knowledge and self-care practices at post intervention and follow-up phases compared to pre-intervention phase with (P