Maha Mamdouh Abdel_Gawad Youssef
Asst. Professor
DNA Polymorphisms and mRNA Levels of Immune Biomarkers as Candidates for Inflammatory Postpartum Disorders Susceptibility in Italian Buffaloes
veterinary science • 2023
Publication Information
Authors
Ahmed Ateya 1,*, Fatmah A. Safhi 2 , Huda El-Emam 1, Muath Q. Al-Ghadi 3 , Mohamed Abdo 4,5 ,
Liana Fericean 6, Rada Olga 6,*, Ostan Mihaela 6, Manar M. Hizam 7, Maha Mamdouh 8, Eman M. Abu El-Naga 9
and Walaa S. Raslan
Keywords
post partum disorder- inflammation-buffalo
Journal
veterinary science
Publisher
Not Available
Volume
Not Available
Issue
Not Available
Pages
Not Available
publication.type
International
Paper Link
Not Available
Supplementary Materials
Not Available
Abstract
Abstract: The immunological genes that may interact with inflammatory postpartum diseases in Italian
buffaloes were examined in this study. A total number of 120 female Italian buffaloes (60 normal
and 60 with inflammatory reproductive diseases) were employed. Each buffalo’s jugular vein was
pierced to get five milliliters of blood. To obtain whole blood and extract DNA and RNA, the blood
was placed within tubes containing sodium fluoride or EDTA anticoagulants. The immunological
(IKBKG, LGALS, IL1B, CCL2, RANTES, MASP2, HMGB1, and S-LZ) genes’ nucleotide sequence
differences between healthy buffaloes and buffaloes affected by inflammatory reproductive diseases
were found by employing PCR-DNA sequencing. According to Fisher’s exact test (p < 0.01), there
were noticeably different probabilities of all major nucleotide changes spreading among buffalo
groups with and without reproductive problems. Buffaloes were significantly more likely to express
the examined genes when they had inflammatory reproductive diseases. The outcomes might
support the significance of these markers’ nucleotide variations and gene expression patterns as
indicators of the prevalence of inflammatory reproductive disorders and provide a workable buffalo
management policy
buffaloes were examined in this study. A total number of 120 female Italian buffaloes (60 normal
and 60 with inflammatory reproductive diseases) were employed. Each buffalo’s jugular vein was
pierced to get five milliliters of blood. To obtain whole blood and extract DNA and RNA, the blood
was placed within tubes containing sodium fluoride or EDTA anticoagulants. The immunological
(IKBKG, LGALS, IL1B, CCL2, RANTES, MASP2, HMGB1, and S-LZ) genes’ nucleotide sequence
differences between healthy buffaloes and buffaloes affected by inflammatory reproductive diseases
were found by employing PCR-DNA sequencing. According to Fisher’s exact test (p < 0.01), there
were noticeably different probabilities of all major nucleotide changes spreading among buffalo
groups with and without reproductive problems. Buffaloes were significantly more likely to express
the examined genes when they had inflammatory reproductive diseases. The outcomes might
support the significance of these markers’ nucleotide variations and gene expression patterns as
indicators of the prevalence of inflammatory reproductive disorders and provide a workable buffalo
management policy