Maha Mamdouh Abdel_Gawad Youssef
Asst. Professor
Combining Nucleotide Sequence Variants and Transcript Levels of Immune and Antioxidant Markers for Selection and Improvement of Mastitis Resistance in Dromedary Camels
agriculture • 2023
Publication Information
Authors
Ahmed Ateya 1,*, Fatmah A. Safhi 2, Huda El-Emam 1, Marawan A. Marawan 3, Hayat Fayed 4, Amgad Kadah 5,
Maha Mamdouh 5, Manar M. Hizam 6, Muath Q. Al-Ghadi 7, Mohamed Abdo 8,9, Liana Fericean 10,
Rada Olga 10,* and Ostan Mihaela
Keywords
immunity; antioxidant biomarkers; transcript levels; mastitis; dromedary camels
Journal
agriculture
Publisher
MDPI
Volume
13
Issue
1909
Pages
1-18
publication.type
International
Paper Link
Not Available
Supplementary Materials
Maha Mamdouh Abdel_Gawad Youssef_3.pdf
Abstract
The immune and antioxidant genetic factors that could converse with mastitis susceptibility
in dromedary camels were looked at in this research. Of 120 female dromedary camels (60
healthy, and 60 with mastitis) were utilised. Each camel's jugular vein was pierced to obtain five
millilitres of blood. The blood was placed within tubes containing sodium fluoride or EDTA anticoagulants
to obtain whole blood and extract DNA and RNA. The immunological (OTUD3, TLR2,
TLR4, STAB2, MBL2, TRAPPC9, and C4A) and antioxidant (CAT, SOD3, PRDX6, OXSR1, NDUFS6,
SERP2, and ST1P1) genes' nucleotide sequence polymorphisms between healthy and mastitis affected
she-camels were discovered using PCR-DNA sequencing. Fisher's exact test revealed that
camel groups with and without mastitis had noticeably different odds of all major nucleotide alterations
propagating (p < 0.01). Mastitic camels were significantly more likely to express the OTUD3,
TLR2, TLR4, STAB2, MBL2, TRAPPC9, C4A, OXSR1, SERP2, and ST1P1 genes (p < 0.05). However,
CAT, SOD3, PRDX6, and NDUFS6 genes elicited a different pattern. The results may be used to
develop management strategies and support the significance of nucleotide differences and gene
expression patterns in these markers as indicators of the incidence of mastitis.
in dromedary camels were looked at in this research. Of 120 female dromedary camels (60
healthy, and 60 with mastitis) were utilised. Each camel's jugular vein was pierced to obtain five
millilitres of blood. The blood was placed within tubes containing sodium fluoride or EDTA anticoagulants
to obtain whole blood and extract DNA and RNA. The immunological (OTUD3, TLR2,
TLR4, STAB2, MBL2, TRAPPC9, and C4A) and antioxidant (CAT, SOD3, PRDX6, OXSR1, NDUFS6,
SERP2, and ST1P1) genes' nucleotide sequence polymorphisms between healthy and mastitis affected
she-camels were discovered using PCR-DNA sequencing. Fisher's exact test revealed that
camel groups with and without mastitis had noticeably different odds of all major nucleotide alterations
propagating (p < 0.01). Mastitic camels were significantly more likely to express the OTUD3,
TLR2, TLR4, STAB2, MBL2, TRAPPC9, C4A, OXSR1, SERP2, and ST1P1 genes (p < 0.05). However,
CAT, SOD3, PRDX6, and NDUFS6 genes elicited a different pattern. The results may be used to
develop management strategies and support the significance of nucleotide differences and gene
expression patterns in these markers as indicators of the incidence of mastitis.