Tomato yellow leaf curl disease is a major limitation in agriculture across the world, affecting the production of
many crops, resulting in significant economic losses. In Egypt, the incidence of infection is very high, with
visual estimates of TYLCV infection reaching 100 % in some areas. Here in the mechanical transmission ability
of TYLCV is assessed in different plant species well known to be hosts for the TYLCV. The current finding
revealed the failness of transmission through mechanical route. None of the mechanically infected plants
developed TYLC disease symptoms. Furthermore, the absence of TYLCV genome within mechanically
transmitted plants was also validated. Alignment of the amplified partial TYLCV genome from the naturally
infected plants from Qalyubia governorate, show 98.99% similarity and 100% query coverage to the TYLCV in
the GenBank. In conclusion, the TYLCV can’t transmit through mechanical route this may be a result of failing
its genome to be establish within plant genome.