In numerous biomedical applications, including pharmaceuticals, scaffolds for tissue regeneration, drug delivery systems, and imaging agents, natural polymers have been extensively exploited. They serve as templates for regeneration and dressings for either acute or chronic wounds in wound care. There are numerous natural sources of polymers, including plants, animals, and microorganisms. Natural polymer-based scaffolds are desirable for skin repair and regeneration due to their homology to the extracellular matrix, mechanical tunability, high biocompatibility, and high water-holding capacity. An overview of popular or promising natural polymers for wound healing will be provided in this chapter. An overview of popular or promising natural polymers for wound healing will be provided in this chapter.