Sabah Abo-El-maaty Ahmed alsayed
Dept. Head
Identification of Biochemical and Molecular Markers in Tomato Yellow Leaf Curl Virus Resistant Tomato Species.
Scientia Agriculturae • 2013
Publication Information
Authors
Noha K.El-Dougdoug,Sherin A.Mahfouze,Sabah A. Ahmed, Badawi A. Othman, Mahmoud M. Hazaa
Keywords
Symptoms, Peroxidase, polyphenol oxidase, RAPD-PCR
Journal
Scientia Agriculturae
Publisher
© PSCI Publications
Volume
2
Issue
2
Pages
46-53
publication.type
International
Paper Link
Open Link
Supplementary Materials
Not Available
Abstract
Tomato yellow leaf curl virus (TYLCV) is devastating to tomato (Solanum lycopersicum) crops and resistant cultivars are highly effective in controlling the disease. Breeding programmes aimed at producing
TYLCV-resistant tomato cultivars have utilized resistance sources derived from wild tomato species. So far, all reported breeding programmes have introgressed TYLCV resistance from a single wild tomato source. Here, we tested degree of resistant different nine tomato species to TYLCV.Results showed that S. chilense, S. peruvianum, S. hirsutum and S.minutum appeared resistant to TYLCV. On the other hand, the maximum PPO and POD isozyme activities were recorded in the susceptible plants of S. penneleii (15) unique isoforms, while the minimum in the resistant plants of S. minutum (one). Random amplified polymorphism DNApolymerase chain reaction (RAPD-PCR) using five primers revealed the differences between the TYLCV resistant wild tomato species and the healthy control, thus the TYLCV resistant S. hirsutum revealed the highest number with 14 unique markers, followed by S. cheesmaniae with 11markers, while S. chilense and S. peruvianum revealed six markers. These polymorphic fragments are valuable for future use in tomato breeding programs, as marker assisted selection by transfer TYLCV-tolerance genes from a wild green-fruited tomato species to a cultivated line.
TYLCV-resistant tomato cultivars have utilized resistance sources derived from wild tomato species. So far, all reported breeding programmes have introgressed TYLCV resistance from a single wild tomato source. Here, we tested degree of resistant different nine tomato species to TYLCV.Results showed that S. chilense, S. peruvianum, S. hirsutum and S.minutum appeared resistant to TYLCV. On the other hand, the maximum PPO and POD isozyme activities were recorded in the susceptible plants of S. penneleii (15) unique isoforms, while the minimum in the resistant plants of S. minutum (one). Random amplified polymorphism DNApolymerase chain reaction (RAPD-PCR) using five primers revealed the differences between the TYLCV resistant wild tomato species and the healthy control, thus the TYLCV resistant S. hirsutum revealed the highest number with 14 unique markers, followed by S. cheesmaniae with 11markers, while S. chilense and S. peruvianum revealed six markers. These polymorphic fragments are valuable for future use in tomato breeding programs, as marker assisted selection by transfer TYLCV-tolerance genes from a wild green-fruited tomato species to a cultivated line.