The main goal of this research was to see how nanoencapsulation affected the stability and
vitality of the probiotic bacteria in a simulated gastrointestinal digesting and temperature
environment. The mechanical encapsulation approach was used to encapsulate probiotics using
sodium alginate and pullulan. Further, scanning electron microscopy (SEM) was used to
examine the interaction between the capsule matrix and probiotic bacteria in the created nanocapsules. Probiotic bacteria's survival and stability were tested in simulated gastrointestinal,
intestinal, thermal, and refrigeration conditions. Nano encapsulation has a considerable impact
on probiotic bacteria survival and stability. The viability of probiotic bacteria decreased at a
similar rate across all treatments. In contrast, free probiotic cells were shown to have a quick decline in CFU/gram when held at 4oC.Not only did nano-encapsulated bacteria have a lower
reduction in total viable cells than free bacterial cells in an in vitro gastrointestinal assay, but
the results of the viable count in the case of nano-encapsulated cells were also above the
recommended level (106 CFU/gram) under the thermal and simulated GIT conditions.