Samar Mohamed mahmoud mostafa
Lecturer
Molecular and serological data supporting the role of Q fever in abortions of sheep and goats in northern Egypt
Microbial Pathogenesis • 2018
Publication Information
Authors
Abdelfattah Selima,∗
, Abdel-Fattah Alib
, Samar M. Moustafac
, Eman Ramadand
Keywords
Not Available
Journal
Microbial Pathogenesis
Publisher
Elsevier Ltd
Volume
Volume 125
Issue
December 2018
Pages
Pages 272-275
publication.type
International
Paper Link
Open Link
Supplementary Materials
Not Available
Abstract
Q fever is a worldwide zoonotic disease, caused by Coxiella burnetii (C. burnetii), an obligate intracellular bacterium. The epidemiological data about the Q fever situation in Egypt is limited.
The present study investigated the seroprevalence of Q fever among small ruminants in some localities in the northern Egypt and reported the shedders using specific real-time PCR (Rt-PCR). A total of 190 sera and vaginal swabs (110 sheep and 80 goats) were collected from aborted cases. Indirect ELISA was used to detect specific antibodies against C. burnetii, and Rt-PCR was used to detect DNA in the shedder animals. The study revealed that infection was significantly higher in sheep (22.7%) than in goats (12.5%) (p
The present study investigated the seroprevalence of Q fever among small ruminants in some localities in the northern Egypt and reported the shedders using specific real-time PCR (Rt-PCR). A total of 190 sera and vaginal swabs (110 sheep and 80 goats) were collected from aborted cases. Indirect ELISA was used to detect specific antibodies against C. burnetii, and Rt-PCR was used to detect DNA in the shedder animals. The study revealed that infection was significantly higher in sheep (22.7%) than in goats (12.5%) (p