It is known that adverse weather conditions can affect driver performance due to reduction in visibility and slippery surface conditions. Lane keeping is one of the main factors that might be affected by weather conditions. Most of the previous studies on lane keeping have investigated driver lane-keeping performance from driver inattention perspective. In addition, the majority of previous lane-keeping studies have been conducted in controlled environments such as driving simulators. Therefore, there is a lack of studies that investigate driver lane-keeping ability considering adverse weather conditions in naturalistic settings. In this study, the relationship between weather conditions and driver lane-keeping performance was investigated using the SHRP2 naturalistic driving data for 141 drivers between 19 and 89 years of age. Moreover, a threshold was introduced to differentiate lane keeping and lane changing in naturalistic driving data. Two lane-keeping models were developed using the logistic regression and multivariate adaptive regression splines (MARS) to better understand factors affecting driver lane-keeping ability considering adverse weather conditions. The results revealed that heavy rain can significantly increase the standard deviation of lane position (SDLP), which is a very widely used method for analyzing lane-keeping ability. It was also found that traffic conditions, driver age and experience, and posted speed limits have significant effects on driver lane-keeping ability. An interesting finding of this study is that drivers have a better lane-keeping ability in roadways with higher posted speed limits. The results from this study might provide better insights into understanding the complex effect of adverse weather conditions on driver behavior.