Benha University

Tamer Ali

Lecturer

Faculty of Science Zoology

Experience

15 Experience
  • Bioconductor tools.

  • ChIP-Seq (chromatin immunoprecipitation followed by sequencing).

  • Bulk transcriptome sequencing (RNA-seq) & single-cell transcriptome sequence (scRNA-seq)

  • Proteomics: SILAC and free label detection.

  • Embryonic, adult stem cells and iPSCs biology.

  • Drosophila chromatin insulators.

  • CRISPR/Cas system for genome editing.

  • Chromosome conformation capture to study genome-wide interactions.

  • Basic molecular biology techniques: Cloning (using restriction enzyme cloning strategy and Gibson assembly), site-directed mutagenesis, different types of PCR, western blot, BAC recombineering and generating stable cell lines.

  • Gene expression tuning via RNA-guided dCas9.

  • Cell signaling pathways.

  • lncRNA biology

  • Mouse and Drosophila Genetics

  • Reversible protein degradation by Auxin-Degron system

  • Allele-specific epigenome