Tamer Mohamed Shehata Salim
Professor
In vitro, induction of salt tolerant potato (Solanum tuberosum L.) Plants with gamma irradiation and characterization of genetic variations through SDS-PAGE and ISSR-PCR analysis
4th International Conference on Biotechnology Applications in Agriculture (ICBAA), Benha University, Moshtohor and Hurghada, 4-7 April 2018, Egypt • 2018
Publication Information
Authors
Dina Y. M. ELHetawy1, M. S. Abd El-Sabour1, M. H. Refaat1,2 and T. M. Salim1,2
Keywords
Gamma radiation, in vitro mutagenesis, mutation breeding, potato, ISSR, SDS-PAGE, salt stress
Journal
4th International Conference on Biotechnology Applications in Agriculture (ICBAA), Benha University, Moshtohor and Hurghada, 4-7 April 2018, Egypt
Publisher
Faculty of Agriculture, Benha University, Egypt.
Volume
Not Available
Issue
Not Available
Pages
Not Available
publication.type
Local
Paper Link
Not Available
Supplementary Materials
Not Available
Abstract
Salt tolerant mutants of potato (Solanum tuberosum L. ‘Sponta’) were obtained via gamma irradiation. Bud explants of two strains of potato (Sponta and Lady Rosetta were treated with various dosages of gamma irradiation, and the clonal generations were developed. Selection of salt-tolerant mutants was accomplished by in vitro selection media containing 30, 60, 90 and 120 mM NaCl. Molecular-level differences between the control and mutant plants were elucidated using ISSR technique, and the polymorphism rate according to the selected primers was calculated as 89.66%. Genetic distances between the controls and mutants were also calculated, and related dendrograms were produced. On average the mutants were genetically 27.5% different from the control plants. The greatest difference encountered between the control and mutants was 47%, which was detected in mutant plants produced by 20 or 30 Gy gamma irradiation and regenerated in selection medium containing 100 mM NaCl.