Background: Frailty is considered as a complex concept involving a variety of physical, mental, nutritional, and social aspects that are linked to adverse health outcomes in older adults. Aim: This study aimed to evaluate the effect of health promotion intervention on daily living activities among older adults with frailty. Design: A quasi- experimental design was utilized. Setting: The study was carried out at Medicine and Cardiology Outpatient clinics at Benha Teaching Hospital at Benha City in Egypt. Sampling: A purposive sample of 133 older adults was included. Tools: Three tools were used in this study; I: An interviewing questionnaire composed of four parts as older adults’ socio-demographic characteristics, medical history, older adults’ knowledge about frailty and older adults reported practices for health promotion. II: Frailty scale to assess frailty degree among older adults. III: Barthel index scale to assess older adults’ activities of daily living. Results: There was a highly significant improvement in the older adults’ total levels of knowledge, reported practices and dependency levels of activities of daily living post and after three months of health promotion intervention. Also, there was a highly statistically significant correlation between total reported practices, dependency levels of activities of daily living and frailty degree among older adults pre, post and after three months of health promotion intervention. Conclusion: Health promotion intervention was efficient in improving frail older adults’ knowledge, reported practices, and enhancing dependency levels of activities of daily living. Recommendations: Developing continuous health promotion interventions for older adults to enhance their knowledge and practices regarding prevention of frailty.