Background: Artificial intelligence in healthcare has opened new avenues for enhancing patients care, optimizing healthcare operations, and advancing public health initiatives. Aim of study was to assess the perception of nursing students regarding artificial intelligence in health care. Research design: A descriptive research design was utilized to conduct this study. Setting: The study was conducted at Faculty of Nursing, Benha University, Benha City, Egypt. Sample: Simple random sample was used; the total sample included 300 students. Tools: Two online tools were used, Tool I: A questionnaire which included 2 parts: Part I: A): It was concerned with demographic characteristics of nursing students and their technology use. B): It was concerned with the knowledge of nursing students regarding artificial intelligence in health care. Tool II): It was concerned with the attitude of nursing students regarding artificial intelligence in health care. Results: 28.7% of studied nursing students had good knowledge about artificial intelligence in health care, while 36.0% of them had positive attitude regarding artificial intelligence in health care. Conclusion: Approximately two fifths of studied nursing students had average knowledge regarding artificial intelligence in health care, while almost two thirds of them had negative attitude regarding artificial intelligence in health care. Also, there was a positive correlation between nursing students` total knowledge level and their total attitude level regarding artificial intelligence in health care. Recommendations: Health educational programs should be developed and implemented for nursing students to enhance their perception regarding artificial intelligence in health care.