Zeinab Reda Ahmed Abo Ezz
Lecturer
A Recent Non cytopathogenic bovine viral diarrhea virus detected by immunofluorescence and reverse transcriptase polymerase chain reaction
Benha Veterinary Medical Journal • 2021
Publication Information
Authors
Samia Abdelkareem, Ayman Said El-Habbaa, Zeinab RA Aboezz, Ehab ElNahas
Keywords
Not Available
Journal
Benha Veterinary Medical Journal
Publisher
Benha University; Faculty of Veterinary Medicine
Volume
41
Issue
1
Pages
39-41
publication.type
Local
Paper Link
Open Link
Supplementary Materials
Not Available
Abstract
In the present study, a non-cytopathogenic bovine viral diarrhea virus (BVDV) field strains
from bovine semen samples were detected by immunofluorescence (IF) and reverse
transcriptase polymerase chain reaction (RT-PCR). Two out of five semen samples tested
positive for the presence of BVDV antigen employing antigen capture ELISA kits. The two
semen samples were injected in Madin-Darby bovine kidney (MDBK) cells in a trial for
BVDV isolation. The chosen two samples were not cytopathic and subjected for further
identification and genotyping using one step reverse transcriptase polymerase chain reaction
(RT-PCR) assay. The isolated BVDV field strains from semen were found to be BVDV
genotype 1.
from bovine semen samples were detected by immunofluorescence (IF) and reverse
transcriptase polymerase chain reaction (RT-PCR). Two out of five semen samples tested
positive for the presence of BVDV antigen employing antigen capture ELISA kits. The two
semen samples were injected in Madin-Darby bovine kidney (MDBK) cells in a trial for
BVDV isolation. The chosen two samples were not cytopathic and subjected for further
identification and genotyping using one step reverse transcriptase polymerase chain reaction
(RT-PCR) assay. The isolated BVDV field strains from semen were found to be BVDV
genotype 1.