Bovine ephemeral fever virus (BEFV) is an arthropod-borne virus. BEFV causes a
debilitating disease in cattle and water buffaloes. It is enzootic in bovine populations in Egypt
causing great economic losses in animal wealth. Vaccination against BEFV is an important
factor for controlling the disease. Many studies were applied to improve the locally produced
vaccine using different adjuvants in formulation of BEF vaccine. This study aimed to prepare
and evaluate potency of an inactivated BEF vaccine with MontanideTM Gel-01 adjuvant using
serum neutralization test and ELISA. The pattern of humeral immune response to the
prepared vaccine was compared with that of the local inactivated BEF vaccine with
aluminum hydroxide gel adjuvant and live attenuated BEF vaccine inactivated just before
inoculation by saponin. The obtained results revealed that best vaccine was 20% Montanide
Gel 01TM inactivated BEF vaccine, As it give higher antibody titer throughout the experiment
that extend for more than one year post vaccination compared with that of other vaccinated
groups, followed by BEF vaccine inactivated with saponin and 15% MontanideTM Gel-01
based BEF vaccine . Whereas, aluminum hydroxide gel; 5% and 10% MontanideTM Gel01were poor inducer for humeral immune response. So 20% MontanideTM Gel-01based
inactivated BEF vaccine could be recommended in BEF vaccination program.