Obstetric fistula is a major public health issue among thousands of women within a developing country and has the most devastating effects on physical, social, and economic levels. Also, obstetric fistula is a preventable condition that healthcare workers play a key role in its prevention. Aim: The present research was aimed to evaluate the effect of health educational program on maternity nurses' performance regarding obstetric fistula and its prevention. Design: A quasi-experimental research design was utilized. Settings: The research was conducted at Obstetrics and Gynaecology department and emergency department affiliated to Benha University Hospital. Sample: A convenient sample of 80 maternity nurses was recruited. Tools: Four tools were used for data collection; a structured self-administered questionnaire, maternity nurses' knowledge assessment sheet, modified Likert scale for maternity nurses' attitudes and checklists about fistula care skills to assess nurses' practices during caring women with obstetric fistula. Results: Illustrated that, there was a highly statistically significant improvement in relation to maternity nurses’ knowledge, attitudes and practices regarding obstetric fistula and its prevention at post-intervention phase compared to pre-intervention phase (P ≤ 0.001). As well, there was a highly positive statistically significant correlation between total knowledge,