Recently endometriosis is recognized as a challenging problem in gynecology and most common cause of infertility in young women representing 1 in 10 adolescents and women of reproductive age. Raising awareness of endometriosis in adolescence is receiving increasing attention now. Aim: The present research was aimed to evaluate the effect of self-instructional module on adolescent nursing students' awareness regarding endometriosis as challenge for prevention of future infertility. Design: A quasi-experimental research design was adopted to fulfill the aim of this study. Setting: The study was conducted at faculty of nursing, Benha University. Sample: A purposive sample of three hundred and sixty four adolescent female nursing students. Tools: Three main tools: A Structured self-administered questionnaire, Student's awareness assessment sheet regarding endometriosis and Student’s satisfaction sheet.