Ahmed Reda Mohamed Hassan El-Khawagah
Professor
Effect of Adding Quercetin in Dimitropoulos Extender on the Sperm Characteristics and Biochemical Parameters of Arabian Stallion Semen stored at 5 °C
Egyptian Journal of Veterinary Sciences • 2026
Publication Information
Authors
Islam M. Abd El-Fattah, Mohamed M.M. Kandiel, Abdelbary Prince Ahmed R.M. Elkhawagah
Keywords
Arabian Stallion, Chilled semen, Dimitropoulos, Quercetin, Spermiogram.
Journal
Egyptian Journal of Veterinary Sciences
Publisher
Egyptian Journal of Veterinary Sciences
Volume
57
Issue
10
Pages
249-256
publication.type
Local
Paper Link
Open Link
Supplementary Materials
Not Available
Abstract
The present study aimed to determine the effect of adding Quercetin in Dimitropoulos (DM)
extender on the Sperm characteristics and biochemical parameters of equine semen at 5 °C.
Three fertile Arabian stallions were used in this study continued for eight consecutive weeks (from
August to September 2021). Semen collected twice weekly (n= 48) by Missouri artificial vagina was
aliquoted and diluted with DM extenders containing different concentrations of Quercetin (0 µM
(control), 25 µM, 50 µM, and 100 µM) to a final concentration of 25×106 sperm/ml. All groups were
kept at 5 ºC and evaluated daily (every 12 hours) for three days for motility, livability, total
abnormality, metabolic enzymes (ACP, ALP, AST), lactate dehydrogenase (LDH), acrosine activity,
antioxidants (CAT, SOD, GPx), and lipid peroxidation (MDA). The results revealed that Quercetin at
a concentration of 50 µM has an improving effect on sperm motility at 36 and 48 hrs post-chilling. In
addition, it lowered extracellular ACP and enhanced catalase enzyme activities, and lowered MDA
accumulation throughout the experiment. In conclusion, adding quercetin at a concentration of 50
µM has a potential protective effect on sperm motility and decreases oxidation-reduction during cold
storage of stallion semen.
extender on the Sperm characteristics and biochemical parameters of equine semen at 5 °C.
Three fertile Arabian stallions were used in this study continued for eight consecutive weeks (from
August to September 2021). Semen collected twice weekly (n= 48) by Missouri artificial vagina was
aliquoted and diluted with DM extenders containing different concentrations of Quercetin (0 µM
(control), 25 µM, 50 µM, and 100 µM) to a final concentration of 25×106 sperm/ml. All groups were
kept at 5 ºC and evaluated daily (every 12 hours) for three days for motility, livability, total
abnormality, metabolic enzymes (ACP, ALP, AST), lactate dehydrogenase (LDH), acrosine activity,
antioxidants (CAT, SOD, GPx), and lipid peroxidation (MDA). The results revealed that Quercetin at
a concentration of 50 µM has an improving effect on sperm motility at 36 and 48 hrs post-chilling. In
addition, it lowered extracellular ACP and enhanced catalase enzyme activities, and lowered MDA
accumulation throughout the experiment. In conclusion, adding quercetin at a concentration of 50
µM has a potential protective effect on sperm motility and decreases oxidation-reduction during cold
storage of stallion semen.