أميرة حسين محمد علام
أستاذ
Efflux pumps encoding genes (adeA and adeS) in relation to antibiotic resistance pattern in Acinetobacter baumannii strains isolated from Benha university hospital
• 2023
معلومات البحث
المؤلفون
Amira E. Ramadana, Ahmed S. Elgazarb, Nashwa A. Aminc, Amira H. Allamc,
Mai A. Elmahdyd, Naglaa A. B. Eldeena, Sara A. Saiede, Doaa A. Shakera
الكلمات المفتاحية
Not Available
المجلة العلمية
Not Available
الناشر
Not Available
المجلد
Not Available
العدد
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الصفحات
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publication.type
Local
رابط البحث
Not Available
المواد المرفقة
Not Available
الملخص
Background
Acinetobacter baumannii become a growing problem in hospitals as a predominant
multidrug-resistant (MDR). This resistance capacity is generated by various
mechanisms including efflux pumps that can direct antibiotics outwards and
prevent antibiotics from affecting the bacteria.
Methods
Clinical samples were cultured on blood agar and MacConkey agar medium,
identified by Gram stain and biochemical reactions, and then identified to the
species level by Vitek2 automated system. The adeA and adeS genes among
isolated strains were detected by conventional PCR. This study included 50
A. baumannii strains to assess the antibiotic resistance pattern of A. baumannii.
Results
A. baumannii strains were fully resistant to Piperacillin (100%) and lowest resistance
to Imipenem (54%) was observed. Out of 50 isolates, 41 (82%) of A. baumanii
isolates had adeA gene and 32 (64%) had adeS gene. The clinical strains that had
adeA gene and adeS gene showed significantly higher resistance to Ciprofloxacin
(92.7%) and (100%), respectively. Additionally, borderline significant differences
were reported regarding Gentamycin (P = 0.065), Ceftazidime (P = 0.08), and
Meropenem (P = 0.08), with the resistance being higher in the adeA positive group.
Acinetobacter baumannii become a growing problem in hospitals as a predominant
multidrug-resistant (MDR). This resistance capacity is generated by various
mechanisms including efflux pumps that can direct antibiotics outwards and
prevent antibiotics from affecting the bacteria.
Methods
Clinical samples were cultured on blood agar and MacConkey agar medium,
identified by Gram stain and biochemical reactions, and then identified to the
species level by Vitek2 automated system. The adeA and adeS genes among
isolated strains were detected by conventional PCR. This study included 50
A. baumannii strains to assess the antibiotic resistance pattern of A. baumannii.
Results
A. baumannii strains were fully resistant to Piperacillin (100%) and lowest resistance
to Imipenem (54%) was observed. Out of 50 isolates, 41 (82%) of A. baumanii
isolates had adeA gene and 32 (64%) had adeS gene. The clinical strains that had
adeA gene and adeS gene showed significantly higher resistance to Ciprofloxacin
(92.7%) and (100%), respectively. Additionally, borderline significant differences
were reported regarding Gentamycin (P = 0.065), Ceftazidime (P = 0.08), and
Meropenem (P = 0.08), with the resistance being higher in the adeA positive group.