Gehad Sherif Refaat Fahmy Mahmoud El-Assass
Asst. Lecturer
The Possible Ameliorating Effect of Lactoferrin as Adjuvant to Ceftriaxone on Induced Intestinal Sepsis in Adult Male Albino Rats (Histological and Immunohistochemical Study)
• 2025
Publication Information
Authors
Gehad S. Refaat , Mohamed M. Zaki, Helpies D. Shenouda,
Heba E. Bayoumi
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publication.type
Local
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Abstract
Background: Intra-abdominal infections can be seriously
complicated to abdominal sepsis causing injury of various organs
in the abdomen. Lactoferrin could be utilized in combination with
Ceftriaxone to treat intestinal sepsis. Objective: to assess the
possibility of Lactoferrin to ameliorate induced intestinal sepsis
in adult male albino rats when used in combination with
Ceftriaxone. Materials and methods: Fifty male rats were
utilized in this study. The rats were divided into five groups:
Group I (Control), Group II (Abdominal Sepsis model group):
Rats received a single intraperitoneal injection of 1ml fresh fecal
suspension (FSI) at a 50 mg/ml concentration. Group III
(Abdominal Sepsis + Ceftriaxone group): Rats were treated with
an intramuscular injection of Ceftriaxone, Group IV (Abdominal
Sepsis + Lactoferrin group): Rats were treated with oral
Lactoferrin, Group V (Abdominal Sepsis + Ceftriaxone +
Lactoferrin group): Rats were treated with a combination of both.
Colon samples were obtained and processed for histological
examination. Results: Group II exhibited epithelial injury,
detachment, and shedding; some crypts were damaged,
obliterated, and lost parallelism; there was obvious depletion of
goblet cells; and the lamina propria was markedly infiltrated with
inflammatory cells. Furthermore, a significant increase in TNF-α,
COX-2, and Caspase-3 immunoexpression was detected. Groups
III and IV exhibited some histological attenuation, while group V
showed histological architecture with more improvement.
complicated to abdominal sepsis causing injury of various organs
in the abdomen. Lactoferrin could be utilized in combination with
Ceftriaxone to treat intestinal sepsis. Objective: to assess the
possibility of Lactoferrin to ameliorate induced intestinal sepsis
in adult male albino rats when used in combination with
Ceftriaxone. Materials and methods: Fifty male rats were
utilized in this study. The rats were divided into five groups:
Group I (Control), Group II (Abdominal Sepsis model group):
Rats received a single intraperitoneal injection of 1ml fresh fecal
suspension (FSI) at a 50 mg/ml concentration. Group III
(Abdominal Sepsis + Ceftriaxone group): Rats were treated with
an intramuscular injection of Ceftriaxone, Group IV (Abdominal
Sepsis + Lactoferrin group): Rats were treated with oral
Lactoferrin, Group V (Abdominal Sepsis + Ceftriaxone +
Lactoferrin group): Rats were treated with a combination of both.
Colon samples were obtained and processed for histological
examination. Results: Group II exhibited epithelial injury,
detachment, and shedding; some crypts were damaged,
obliterated, and lost parallelism; there was obvious depletion of
goblet cells; and the lamina propria was markedly infiltrated with
inflammatory cells. Furthermore, a significant increase in TNF-α,
COX-2, and Caspase-3 immunoexpression was detected. Groups
III and IV exhibited some histological attenuation, while group V
showed histological architecture with more improvement.