هبه محمد عبد الكريم على
أستاذ مساعد
Urinary NDRG2 and MCM8 Gene Expression as New Noninvasive Biomarkers for Diagnosis and Differentiation between Muscle and Non-Muscle Invasive Bladder Cancer
Biomedical and Pharmacology Journal • 2023
معلومات البحث
المؤلفون
Heba M. Abdel Kareem1,2, Aiman I. Al-Qtaitat3,4, Fadi S. Sawaqed5 and Fardous S. Karawya3
الكلمات المفتاحية
Bladder Carcinoma; Mini Chromosome Maintenance Proteins; NDRG-2;
Polymerase Chain Reaction; Voided Urine Cytology.
المجلة العلمية
Biomedical and Pharmacology Journal
الناشر
Not Available
المجلد
Not Available
العدد
Not Available
الصفحات
Not Available
publication.type
International
رابط البحث
Not Available
المواد المرفقة
Not Available
الملخص
Noninvasive diagnosis of cancer bladder remains a challenge. The study aimed to
evaluate the urinary gene expression of NDRG-2 (N-Myc downstream-regulated gene2) and
MCM8 (the mini chromosome maintenance proteins) genes and their importance as novel urinary
biomarkers for bladder cancer. In addition, to assess their diagnostic value in comparison with
voided urine cytology is the focus of this work. the study included twenty healthy controls and
fifty patients with bladder cancer. Quantitative real-time polymerase chain reaction (qRT-PCR)
and voided urine cytology (VUC) were performed to demonstrate the NDRG2 and MCM-8 gene
expression levels in the urine of healthy controls and bladder cancer patients. There was a
statistically significant decrease in NDRG-2 gene expression in bladder cancer group (4.38±0.66)
compared to the control group (8.29±1.67). Gene expression of MCM-8 showed a statistically
significant increase in bladder cancer group (5.57±0.79) in comparison to control group
(4.55±1.39) with a significant negative correlation (?= -0.77) between NDRG-2 expression levels
and tumor grade in cancer group (p
evaluate the urinary gene expression of NDRG-2 (N-Myc downstream-regulated gene2) and
MCM8 (the mini chromosome maintenance proteins) genes and their importance as novel urinary
biomarkers for bladder cancer. In addition, to assess their diagnostic value in comparison with
voided urine cytology is the focus of this work. the study included twenty healthy controls and
fifty patients with bladder cancer. Quantitative real-time polymerase chain reaction (qRT-PCR)
and voided urine cytology (VUC) were performed to demonstrate the NDRG2 and MCM-8 gene
expression levels in the urine of healthy controls and bladder cancer patients. There was a
statistically significant decrease in NDRG-2 gene expression in bladder cancer group (4.38±0.66)
compared to the control group (8.29±1.67). Gene expression of MCM-8 showed a statistically
significant increase in bladder cancer group (5.57±0.79) in comparison to control group
(4.55±1.39) with a significant negative correlation (?= -0.77) between NDRG-2 expression levels
and tumor grade in cancer group (p