Some of the significant globally prevalent vector
borne illnesses are caused by Culex pipiens. Synthetic pes
ticides have been widely utilized to eradicate C. pipiens,
which has led to a number of health risks for people, insect
resistance, and environmental contamination. Alternative
strategies are therefore vitally needed. In the current
investigation, the Trichoderma viride fungal culture filtrate
was used to create selenium and silver nanoparticles
(SeNPs and AgNPs, respectively) and test them on C. pipiens
larvae in their fourth instar stage. The death rate increased
significantly when SeNP and AgNP concentrations increased,
according to the results. SeNPs and AgNPs significantly
affected the developmental and detoxification enzymes in
fourth instar larvae of C. pipiens at 24 h after being treated
with the sublethal concentration of the tested NPs. As
a result of their insecticidal effect on C. pipiens larvae,
SeNPs and AgNPs are considered effective and promising
larvicidal agents