Background: Tympanoplasty is defined as surgical procedure to eradicate infection and restore the function of the middle ear. It is the commonest procedure performed in patients with chronic otitis. Aim of the study: The study aimed to evaluate the effect of the nursing instructions on patients’ health outcomes post tympanoplasty. Study design: Quasi-experimental study design was implemented to achieve the aim of the present study. Setting: The present study conducted in otolaryngology department and outpatient clinic at Benha University Hospital. ,Qalyubia , Egypt. Subjects: Purposive sample of 80 adult patients post tympanoplasty, were included in the study Tools of data collection: Four tools were used in the study Tool I Structured interview questionnaire, Tool II patients’ self-care practice assessment Tool III Hearing Handicap Inventory for Adults (HHIA) Scale Tool IV Post operative complications assessment. Results: there was a statistically significant differences regarding basic knowledge about ear drum perforation between pre and post nursing instructions’ implementation, in term of improvement in knowledge level. Where, causes of eardrum perforation and diagnostic methods of eardrum perforation were of the highest areas of correct knowledge post nursing instructions among 90.0% compared to 40.0% & 35.0%, respectively pre instruction implementation, there was a statistically significant differences regarding total patients’ self-care practice after tympanoplasty between pre and post nursing instructions’ implementation, in term of statistical significance improvement in self-care practice level. Where, dealing with the wound after tympanoplasty was of the highest area of satisfactory total self-care practice post nursing instructions among 75.0% compared to 13.8 % pre instruction implementation. Conclusion: patients post tympanoplasty had marked improvement in knowledge and practice score and they had significant statistical reduction in the incidence of complications post nursing instruction which confirmed the study hypotheses.