hoda ashour ahmed desoky
Lecturer
EFFECT OF JATROPHA LEAVES EXTRACT ON IN VITRO DATE PALM EMBRYOS GERMINATION AND SHOOT REGENERATION
Plant Archives • 2023
Publication Information
Authors
Rasha, N. Arafa1,*
, Sayed, A. A. Elsayh2, Ghada, A. Ali1, Marwa M. Abdalgaleel2, I. M. Shams El-Din1,
Salwa El-Habashy2, Emadeldin A.H. Ahmed2, Eman H. Afifi2, Rabab W. El Aramany1, Yasser,
S.G. Abd Elaziz2 and Dosoky, Hoda A.A. 3
Keywords
Date palm, jatropha leaves extract, natural growth additive, somatic embryos and shoot multiplication.
Journal
Plant Archives
Publisher
Dosoky, Hoda A.A. 3
Volume
Vol. 23, No. 2, 2023
Issue
ISSN:0972-5210
Pages
pp.379-388
publication.type
International
Paper Link
Open Link
Supplementary Materials
hoda ashour ahmed desoky_inbound3608202894528015700.pdf
Abstract
The aim of this study was to enhance the shoot production of date palm, Hayani cv. by investigating the effect of
adding jatropha leaves extract in appropriate doses to the germination and multiplication media of date palm. a small
cluster of 7-10 matured somatic embryos of date palm Hayani cv. were grown for 12 weeks on MS medium with 0.5
mg l-1 BA, 0.1 mg l-1 NAA and 10.0 ml l-1 JLE which produced the highest number of somatic embryos (28.3
embryos/jar), with the maximum number of leaves (34.5 leaf/jar) with the longest leaf length (6.0 cm). furthermore;
during shoot multiplication stage, clusters of shoots cultivated on MS basal medium supplemented with 1.0 mg l-1 BA,
0.5 mg l-1 TDZ and 0.25 mg l-1 NAA with 10 ml l-1 JLE resulted in the best shoot regeneration, with maximum
number of shoots (25.2 shoots/jar), the maximum number of leaves (56.0 leaf/jar), and the longest leaf length (5.0
cm). The regenerated shoots with lengths of 5–7 cm were grown on ½ MS medium with 1.0 mg l-1 NAA during the
adding jatropha leaves extract in appropriate doses to the germination and multiplication media of date palm. a small
cluster of 7-10 matured somatic embryos of date palm Hayani cv. were grown for 12 weeks on MS medium with 0.5
mg l-1 BA, 0.1 mg l-1 NAA and 10.0 ml l-1 JLE which produced the highest number of somatic embryos (28.3
embryos/jar), with the maximum number of leaves (34.5 leaf/jar) with the longest leaf length (6.0 cm). furthermore;
during shoot multiplication stage, clusters of shoots cultivated on MS basal medium supplemented with 1.0 mg l-1 BA,
0.5 mg l-1 TDZ and 0.25 mg l-1 NAA with 10 ml l-1 JLE resulted in the best shoot regeneration, with maximum
number of shoots (25.2 shoots/jar), the maximum number of leaves (56.0 leaf/jar), and the longest leaf length (5.0
cm). The regenerated shoots with lengths of 5–7 cm were grown on ½ MS medium with 1.0 mg l-1 NAA during the